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From 2012.igem.org

Electrotransformation

Used Bioline electrocompetent cells.
Dilute Assembly mix (1 µL) in water (3 µL). 1 µL was take and mixed with 40 µL electrocompetent cells.

Defrost cells and immediately add 40 uL to 1 uL of diluted assembly mix. Sit on ice 0.5-1min. Add to 0.1cm electroporation cuvette prechilled on ice. Put in electroporator and electroporate at 1.8kV (hold both buttons down "CHG 9" appears then when you hear the beep the electroporation has occurred.) Immediately add 1 mL of SOC and mix and transfer to 15 mL tube. Grow out for 1 hr at 37 C and plate onto selective media.