Team:Macquarie Australia/Protocols/electrotransformation



Used Bioline electrocompetent cells.
Dilute Assembly mix (1 µL) in water (3 µL). 1 µL was taken and mixed with 40 µL electrocompetent cells.

Defrost cells and immediately add 40 uL to 1 uL of diluted assembly mix. Sit on ice 0.5-1min. Add to 0.1cm electroporation cuvette prechilled on ice. Put in electroporator and electroporate at 1.8kV. Immediately add 1 mL of SOC and mix and transfer to 15 mL tube. Grow out for 1 hr at 37 C and plate onto selective media.