Team:Lyon-INSA/protocol
From 2012.igem.org
Our protocols
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Pouring of plates
Bacillus subtilis Transformation
Storage of strains
Plasmid or Cosmid DNA Purification Using QIAGEN HiSpeed Plasmid Midi Kits
QIAquick PCR Purification Kit
Ligation
TSS chemical transformation
NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli
Ozyme digestion
Tests on staphylococcus aureus biofilms
Tests on Staphylococcus epidermidis biofilms in 24 wells plate
Tests of Bacillus subtilis adherence in 24 wells plate
OD 600nm Test
Filling-in
SDS-PAGE