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Team:KIT-Kyoto/Notebook-week6
From 2012.igem.org
September 11thThe female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. September 12thThe transfected cells were examined under the confocal lazer microscope (Olympus, Fv10i) Results: about 20 % cells were observed to be EGFP-positive, indicating that the P-element plasmid, pUAS-EGFP-TNFAIP3 is functional in Drosophila cells. We therefore decided to microinject this DNA into Drosophila embryos to establish transgenic flies. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. September 13thThe female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. September 14thThe adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. September 15thThe female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. September 16thThe adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. In total 83 flies from microinjected embryos were mated with yw flies. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. September 17thThe progeny flies were inspected by dissecting microscope to look for the successfully transformed w+ red eye flies (red eye screening). However, no red eye fly was found. |
