"

From 2012.igem.org

Protocols  |  Single Gene Plasmids and Enzyme Characterisation  |  Showcasing Polysaccharide Production  |  The 3-Gene Inducible Plasmid Operon Construction  |  Glycobase

Protocols

Transformation Protocol from Invitrogen, for Top10 Competent Cells Transferring Cultures to Liquid Medium Mini Prep New England BioLabs Biobrick Assembly Protocol Annealing Oligomer Primers Biobrick Extraction Two-Step PCR Bacterial Genomic DNA Extraction Using GenElute PCR purification using QIAquick Linearized plasmid backbone digestion and ligation protocol Preparation of IDTE buffer and IDT re-suspension 3-step PCR