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Team:Evry/Data
From 2012.igem.org
Data page: Summary of what we have done this year (so far!)
New frog plasmids
- K812000: PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system
The auxin system
The auxin receiver system
- K812010:GFP fusion with the ubuquitinase E3 OsTirI recoginition domain which a main part of the degron system
- K812012:OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
- K812013:The Auxin reciever system golden gate-assembled containing GFP-AID OsTirI polysistronic system for auxin detection in tadpole
The auxin emission system
- K812020:IAAH enzyme that catalyzes the second and last step towards auxin production
- K812021:IAAM enzyme that catalyses the transformation of Tryptophan to Inodle-3-acetamide
- K812014:Auxin production cassette containg both enzymes above which is monocystronic
Reporters and promotors
- K812030:
Characterization
the information on characterized part can be found herein:
- FrenchFrog:
Goldenbricks
Summary
Fig 4: Summary of the GoldenBrick procedure
The GoldenBrick is a new assembly method for the partsregistry. If you want to know more, see this page.
Our favourites parts
- K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
- K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
The other parts we have created
- K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
- K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
- K812053: A GoldenBricked version of the strong RBS B0034
- K812054: A GoldenBricked version of the RFP E1010
- K812055: A GoldenBricked version of the terminator B0015
- K812056: A GoldenBricked version of the pLac R0010 promoter
- K812057: A GoldenBricked of an sfGFP protein
- K812058: A GoldenBricked of medium strenght RBS J61107
- K812059: A GoldenBricked of week RBS J61117
say we are testing them
- FrenchFrog:
