"

From 2012.igem.org

Auxin Degron System in Iadpoles Our System is based on a ligand-receptor communication where auxin is produced in a two step manner. following production, when auxin interacts with the auxin-inducible degradation(AID), the bound protein to the AID is thereafter degraded. In our system the AID-bound protein is the famous GFP reporter which upon loss of fluorescence, indicates the system's fidelity ).

The following Diagram is a schematic representation of the Auxin-sensing System' along with the main necessary interacting parts in the system submitted by our team:

| Data For Our Favorite New Parts

1. [http://partsregistry.org/Part:BBa_K812000 Main Page] - Eukaryotic exoression vector for tadpole injections , BBa_K812000: This vector has all the prerequisites for eukaryotic trasncriptiona like 5' and 3' Untranslated regions implicated in effective transcriptional regulations . On top of this, it has all the requirements for amplification of any casette in bacteria.

1. [http://partsregistry.org/Part:BBa_BBa_K812000 Main Page] - Auxin production cassette, BBa_K812000: The Ligand Production Cassette

Auxin Production System'

# [http://partsregistry.org/Part:BBa_K812013 Main Page] - BAR Bad Odor Receptor, BBa_K812013: The Auxin -induced degradation system of GFP which includes the GFP fused with AID upstream to OSTir1 which upon expression they form the auxin degradation system. Consequently, after interaction of auxin GFP will be degraded

Auxin-Induced Degradation System Response Cassette

|- | Golden Brick Parts

1. [http://partsregistry.org/Part:BBa_J45119:Experience Experience] - Wintergreen odor enzyme generator, BBa_J45119 (MIT, iGEM 2006): 98 out of 100 volunteer subjects standing up to 5 feet away from the bacterial cultures could distinguish wintergreen-producing bacteria from negative controls.
2. [http://partsregistry.org/Part:BBa_J61110:Experience Experience] - RBS, BBa_J61110 (Arkin Lab, 2007): Of the 5 RBS Parts we tested, this RBS works best for expressing yellow fluorescent protein-tagged BAR

| Text bubble 3 |- | We've Also Characterized the Following Parts

1. [http://partsregistry.org/Part:BBa_XXXXX Main Page] - Air Freshilizor, BBa_XXXXX: Our mathematical model predicts that the threshold of activation is 10 parts per billion, the concentration of Butanethiol that humans can typically smell

| Text bubble 4 |}

In this example, this fictional team ran out of time before they could test the complete device and use it as a synthetic air freshener. However, they made progress testing their new promoter by using GFP and they did some mathematical modeling to understand the behavior of the system. They also conducted a test to see how far the wintergreen scent could travel from a flask of culture.