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From 2012.igem.org

Contents 1 Results 1.1 The Constructor 1.2 Plug and Apply System 1.3 Outside Modifications 1.4 Inside Modifications 1.5 Virus Like particles 1.6 General

Results

The Constructor

More efficient cloning, publication, importance of computational work in SynBio

iGEM 2011 team	iGEM project title	# of TU's in the designed circuit	# of used Biobrick Parts By team	Recommended by the Constructor	Processing time (min)
KAIST-Korea1	E. casso	2	11	7	< 1 min
UANL_Mty-Mexico2	S.C.I.E.N.C.E. : Simple Code Interpretation Enabling Circuit in E. coli	7	26	25	674 min
XMU-China3	i-ccdB: intelligent Control of Cell Density in Bacteria	3	6	6	< 1 min
NTNU-Trondheim4	Red Fluorescent Stress Sensor	2	5	4	< 1 min
Wageningen UR5	The Synchroscillator: a Synchronized Oscillatory System	4	7	6	< 2 min

1 https://2011.igem.org/Team:KAIST-Korea

2 https://2011.igem.org/Team:UANL_Mty-Mexico

3 https://2011.igem.org/Team:XMU-China

4 https://2011.igem.org/Team:NTNU_Trondheim

5 https://2011.igem.org/Team:Wageningen_UR

Our publication of The Constructor at the Journal of Biological Engineering [Highly Accessed].

The poster of The Constructor, presented at the German Synthetic Biology Conference in May 2012 in Munich.

Plug and Apply System

GFP-coil, coils, the idea

Outside Modifications

• Designed the primers for amplification of the needed constructs
• Delivered the proof of principle that these primers work [link to framshift, Hugo]
• Provided the templates for modifications and placed them in the partsregistry
• Isolated the PLRV from nature which, in theory, should be easy to modify on the outside

Inside Modifications

Negative CCMV, Frameshift

Virus Like particles

Detection Isolation from nature

• RNA isolation
• RT-PCR
• Addition of prefix and suffix
• Transformation of E.coli
• Isolation of plasmids
• Sequencing of PLRV CP's
• Alignment experiments

General

• We organised a mini Symposium for all dutch teams in the begining of the competition to create a national network and eventualy a national effort
• We visited secondary schools to teach them about synthetic biology and let the make leafelets about synthetic biology.
• We went to present our project and The Constructor at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams
• We communicated to a graphical designer to obtain an kinetic graphical movie of our VLP formation, that is included in our introduction page movie.
• We involved a communication study in our discovery festival human practises, so that we do not only inform the public, but also will have results on how scientist communicate with the public.
• We have taken a number of inverviews with stakeholders to have an opinion of expert on our project, regulations around our project and about the idea of an open source environment