No.
| Name
| Firma
| Order-ID
|
1
| Terephthalat
| Sigma-Aldrich
| 185361-500G
|
2
| Acetic acid
| Roth
| 6755.1
|
3
| Methanol
| Roth
| CP43.1
|
4
| Coomassie Brilliant Blue R-250
| Roth
| 9598.1
|
5
| Trypton
| Roth
| 8952.1
|
6
| Ethylene glycol
| Roth
| 9516.1
|
7
| 3,4-Dihydroxybenzoic acid
| Sigma-Aldrich
| 37580-25G-F
|
8
| Catechol
| Sigma-Aldrich
| C9510-100G
|
9
| Isopropyl β-D-1-thiogalactopyranoside
| Roth
| CN08.1
|
10
| Tetramethylethylenediamine
| Roth
| 2367.3
|
11
| Ammonium persulfate
| Roth
| 9592.3
|
12
| Bromophenol blue
| Roth
| A512.1
|
13
| Glucose
| Roth
| X997.1
|
14
| Calcium chloride
| Roth
| CN92.1
|
15
| Dithiothreitol
| Roth
| 6908.1
|
16
| Sodium carbonate
| Roth
| 8563.1
|
17
| Phenylmethanesulfonylfluoride
| Roth
| 6367.1
|
18
| NADH-Disodium
| Roth
| AE12.1
|
19
| Nicotinamide adenine dinucleotide
| Roth
| AE11.1
|
20
| orthophosphoric acid 85%
| Roth
| 9079.1
|
21
| Trichloroacetic acid
| Roth
| 8789.2
|
22
| Quick-Load® 100 bp DNA Ladder
| NEB Biolabs
| N0467S
|
23
| Phenol
| AppliChem
| A4458.0250
|
24
| Dimethyl sulfoxide
| AppliChem
| A1584.0100
|
25
| Methylene blue
| AppliChem
| A4084.0025
|
26
| Magnesium sulfate
| AppliChem
| A6287.0250
|
27
| Cetrimonium bromide
| AppliChem
| A0805.0100
|
28
| Tetramethylethylenediamine
| AppliChem
| A1148.0025
|
29
| Urea
| AppliChem
| A5470.0500
|
30
| Tween20
| AppliChem
| A1389.0500
|
31
| Protein Marker III (6,5-200)
| AppliChem
| A4402.0001
|
32
| Thiamine hydrochloride
| AppliChem
| A09955.0050
|
33
| Triton X-100
| AppliChem
| A1388.0500
|
34
| DNA-Dye NonTox
| AppliChem
| A9555.1000
|
35
| Agarose
| Roth
| 3810.2
|
36
| Ethanol 96 %
| Roth
| T171.4
|
37
| Ethylenediaminetetraacetic acid
| Roth
| X986.2
|
38
| Ethanol
| Roth
| P076.1
|
39
| Tetracycline hydrochloride
| Roth
| 237.1
|
40
| D-Desthiobiotin 10x Buffer E
| iba
| 2-1000-025
|
41
| 2-(4-Hydroxyphenylazo)benzoic acid
| Sigma-Aldrich
| H5126-5G
|
42
| 10 mM dNTP-Mix
| Roth
| L785.2
|
43
| Hydrochloric acid
| Roth
| 9277.1
|
44
| Acrylamid - Bis (37,5:1)
| Roth
| 3029.1
|
45
| Ammonium acetate
| Roth
| 7869.2
|
46
| Agar-Agar
| Roth
| 5210.3
|
47
| Chloroform
| Roth
| 4423.1
|
48
| Glycine
| Roth
| 3790.2
|
49
| Dipotassium phosphate
| Roth
| T875.1
|
50
| Sodium chloride
| Roth
| 9265.1
|
51
| Yeast extract
| Roth
| 2363.2
|
52
| Monopotassium phosphate
| Roth
| P018.1
|
53
| Glycerine
| Roth
| 3783.1
|
54
| Sodium hydroxide
| Roth
| P031.1
|
55
| Tris-Base
| Roth
| AE15.2
|
56
| Isopropyl alcohol
| Roth
| CP41.3
|
57
| Isoamyl alcohol
| Roth
| T870.1
|
58
| Acetonitrile
| Roth
| 7330.2
|
59
| Acetone
| Roth
| 5025.5
|
60
| Chloramphenicol
| Roth
| 3886.2
|
61
| Ampicillin
| Roth
| K029.1
|
62
| Kanamycin
| Roth
| T832.1
|
63
| Sodium dodecyl sulfate
| Roth
| 5136.1
|
No.
| Name
| 5´-->3´
|
1
| tphA2-l-F
| TCATGCTTGCATCTCCTGTC
|
2
| tphA2-l-Prefix
| GAATTCGCGGCCGCTTCTAGATGCAAGAATCCATCATCCAGTGGC
|
3
| tphA1-Suffix_R
| CTGCAGCGGCCGCTACTAGTACTAATGGTTGCCAGTCGGGTCTG
|
4
| tphA3-Suffix_R
| CTGCAGCGGCCGCTACTAGTATCATAGCGGCAATGACATCAGCGTG
|
5
| tphA3-Prefix_F
| GAATTCGCGGCCGCTTCTAGATGATCCATGAAATTCAAATCGCGG
|
6
| tphA1-l-R
| CTAATGGTTGCCAGTCGGGT
|
7
| tphA1-l-PstI(99)-F
| CATGGTCTCCTCCAGGCCGGCATCGAGCT
|
8
| tphA1-l-Prefix
| GAATTCGCGGCCGCTTCTAGATGAACCACCAGATCCATATCCACG
|
9
| tphA3-l-F
| TCATAGCGGCAATGACATCA
|
10
| tphA3-l-Prefix
| GAATTCGCGGCCGCTTCTAGAGATGATCCATGAAATTCAAATCGC
|
11
| Prefix
| GAATTCGCGGCCGCTTCTAGAG
|
12
| tphB-l-F
| TCAGACCGGTTGGGCTCCGA
|
13
| tphB-l-Prefix
| GAATTCGCGGCCGCTTCTAGAGATGACAATAGTGCACCGTAGATT
|
14
| tphA2-l-R
| ATGCAAGAATCCATCATCCAG
|
15
| tphA1-l-R
| ATGAACCACCAGATCCATATC
|
16
| tphA1-l-PstI(99)-R
| CATGGTCTCCTGGAGGGCTGCATCCAGTAC
|
17
| tphA3-l-R
| ATGATCCATGAAATTCAAAT
|
18
| Suffix
| CTGCAGCGGCCGCTACTAGTA
|
19
| tphB-l-Suffix-R
| CTGCAGCGGCCGCTACTAGTATCAGACCGGTTGGGCTCCGAGTA
|
20
| EcoRIGFxa-tphA1
| TTCTGGAATTCGGGTATTGAAGGAAGAATGAACCACCAGATCCATAT
|
21
| EcoRIGFxa-tphA2
| TTCTGGAATTCGGGTATTGAAGGAAGAATGCAAGAATCCATCATCCA
|
22
| EcoRIGFxa-tphA3
| TTCTGGAATTCGGGTATTGAAGGAAGAATGATCCATGAAATTCAAAT
|
23
| EcoRIGFxa-tphB
| TTCTGGAATTCGGGTATTGAAGGAAGAATGACAATAGTGCACCGTAG
|
24
| EcoRIGFxa-aroY
| TTCTGGAATTCGGGTATTGAAGGAAGAATGACAGCGCCTATCCAG
|
25
| RBS-tphA1
| TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGAACCACCAGATCCATAT
|
26
| RBS-tphA2
| TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGCAAGAATCCATCATCCA
|
27
| RBS-tphA3
| TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGATCCATGAAATTCAAAT
|
28
| RBS-tphB
| TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGACAATAGTGCACCGTAG
|
29
| RBS-aroY
| TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGACAGCGCCTATCCAG
|
30
| VF2
| TGCCACCTGACGTCTAAGAA
|
31
| VR
| ATTACCGCCTTTGAGTGAGC
|
32
| pPR-IBA2 rev
| TAGTTATTGCTCAGCGGTGG
|
33
| pPR-IBA2 for
| TAATACGACTCACTATAGGG
|