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From 2012.igem.org

Contents 1 Appendix 1.1 Constructs 1.2 Protocols 1.3 Kits 1.4 Chemicals 1.5 Software

Appendix

To complete this report we list all products and software used over the course of our project.

Constructs

pRS	p1	p2	p3
313	Padh1	mPR danio rerio	Tadh1
313	Padh1	mPR Xenopus laevis	Tadh1
315	Pfet3	rox1	Tadh1
315	Pfet3	mig1	Tadh1
316	Panb1	lacZ	Tadh1
316	Panb1	luciferase	Tadh1
316	Psuc2	lacZ	Tadh1
316	Psuc2	luciferase	Tadh1

Protocols

Kits

• Genaxxon Plasmid DNA Purification Mini Prep
• Genaxxon Gel Extraction Mini Prep
• Genaxxon PCR DNA Purification Mini Prep
• Qiagen Plasmid Midi

Chemicals

We needed the following chemicals:

• Ampicillin
  beta-lactam antibiotic
• Agarose
  Polysaccharide, major component of Agar
• Dimethylsulfoxid (DMSO)
  organic solvent
• Acetic Acid
• Ethylenediaminetetraacetic acid (EDTA)
• Ethanol
• TRIS
  buffer solution for enzymes
• Nucleoside triphosphate
• Trypton
• Isopropanol
• Isopropyl-β-D-thiogalactopyranosid (IPTG)
• LB-medium
  used for growth of E.coli
• Agar-Agar

Software

The following list of software was used in the team:

• [http://ugene.unipro.ru/ Unipro UGENE]
  UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.

• [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial)
  All our primers were designed using Vector NTI which is also used by our advisors.

• [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
  BLAST was our main sequence search tool. It was quite useful for controlling sequence results.

• [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]

• [http://drive.google.com Google Drive]
  Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).