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From 2012.igem.org

week 10: 2 july - 8 july

Office work

Lab work

TuYV

Monday:

• A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa E. Coli was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated.

• Because there is iGEM illegal sites in our TuYV readthrough part, we use a new construct named 4 and 4H, which are coat protein with part of the readthrough without illegal site and coat protein with part of the readthrough without illegal site with his-tag.

• We tried to isolate and amplify 4 and 4H today, but we did not got any bands on the agrose gel check

Tuesday:

• today we successfully PCR amplified the construct 4, which is around 800bp.

• Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts.

Written by: Han Yue

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