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Team:SDU-Denmark/labwork/Protocols/3A
From 2012.igem.org
| mRNA Isolation | PCR | Miniprep | Check Digest |
| 3A-Assembly | Content | Content | Content |
| Content | Content | Content | Content |
3A assembly
Ligation of 2 parts into a vector
- PCR step; see PCR protocol
- Digestion
- Digest step for Plasmid Backbone
BSA is only needed for conventional restriction enzymes, NOT fast digest
