Team:TU Darmstadt/Materials/tctB162

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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB162]

Primers for the construction of a BioBrick containing the small subunit B1 of the tripartite tricarboxylate transporter family.

tctB162 BioBrick

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgaaaatcaagagccaaaaagactttttctc
rev: gtttcttcctgcagcggccgctactagtattagccagcgatgaagctaggcc

tctB162 Pst1

Primers for the elimination of a Pst1 site inside the tctB162 BioBrick.

     5'                             3'
fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC
rev: GGCCACACGGGGAACTGGAGATTCAGCGCC

tctB162 RBS

Primers for the insertion of a RBS based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101].

     5'                                                                                 3'
fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAAAGAGGGGACAAtactag atgaaaatcaagagccaaaaagactttttctc
rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttagccagcgatgaagctaggcc