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Template:Team:TU Munich/Overview
From 2012.igem.org
Contents |
Ethanol Inducible Promoter
The KlADH4-Promoter from the yeast Kluyveromyces lactis regulates the expression of a mitochondrial alcohol dehydrogenase in an ethanol-dependent way.
The upstream activating sequence of the promoter contains the consensus sequence of the of the RAP1 (repressor activator protein 1) binding site as well as 5 stress response elements. Those 6 site are essential to the ethanol inducibility of the promoter and their respective transcription factors are present in K. lactis as well as S. cerevisiae.(4,6)
Light Switchable Promoter
The idea behind a lightswitchable system is to create a gene expression system which can be induced and deactivated by light of a certain wavelengths.
This system is extremely attractive, as induction does not require the addition of a specific substance. This makes induction cheap, fast, precise and also compatible to the bavarian purity law.
Constitutive Promoter
When all enzymes are expressed under control of promoters with the same strength, the enzyme with the lowest kinetic rates will cause a bottleneck in the pathway. Hence a multitude of promoters of different strengths is needed.
