Team:TU Darmstadt/Materials/TAE

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Contents

TAE

About

TAE is a commonly used buffer for agarose gel electrophoresis that provides optimal resolution of fragments >4kb in length.

Application

TAE is usually prepared in 50X stocks that are diluted to 1X when needed.

Ingredients

For 1L 50x:

  • 242 g Tris-HCl/Tris-base
  • 57 mL 100% acetic acid
  • 18,6 g EDTA (Ethylenediaminetetraacetic acid) or 100mL of 0.5M sodium EDTA

Add dH2O up to 1L For 1L 1x:

  • 20ml 50x TAE

Add dH2O up to 1L (980mL)

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