Team:TU Darmstadt/Materials/BsaI

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Contents

BsaI

About

BsaI is a restriction enzyme isolated from [http://en.wikipedia.org/wiki/Bacillus_stearothermophilus Bacillus stearothermophilus 20241].

Working conditions

The recognition motive of BsaI is:[1] 

5' GGTCTC --> 5'---GGTCTCN     NNNN---3'
3' CCAGAG --> 3'---CCAGAGNNNNN     ---5'

Supplier

  • NEB [http://www.neb.com/nebecomm/products/productR0535.asp]

References

[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_Bsa-Bso

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