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From 2012.igem.org

week 5: 4 june - 8 june

office work

Wiki page

Monday:

• Update notebook and protocols
• Start to make master-plan for wiki page
• Developing the wiki template further

This monday we've worked further on updating the wiki, we are now on schedule with the notebook and protocols. We also made a master plan on how the wiki should look like. The first steps are being made to create a prize winning wiki page.

Tuesday:

• Update notebook and protocols
• Develop master-plan further

[Meeting]

written by: Mark

lab work

CCMV Bricking:

Monday:

• Check agar plates for colonies
• Grow colonies in LB medium + kanamycin, checked optical density

Colonies:

1. CCMV-1
2. CCMV-2
3. d26-CCMV-1
4. d26-CCMV-2
5. d26-CCMV-3

• overnight in shaker 37 degree Celcius

The monday morning of this week started with a bit of a shock: our plates with our transformed E.coli we're almost completely empty. Luckely there was at least one colony on both of the plates with transformed E.coli containing bricks. We put them in Greiner tubes with LB-medium and a antibiotic and after a few hours checked their optical density. In the afternoon we prepared the samples for a PCR to validate the bricks.

Tuesday:

• Checked Greiner tubes

Colonies:

1. CCMV-1: growth
2. CCMV-2: no growth
3. d26-CCMV-1: growth
4. d26-CCMV-2: growth
5. d26-CCMV-3: growth

• Mini prep the 4 remaining samples

CCMV test protocol:

Monday:

• Prepared two samples for dialysis according to the dialysis protocol
• Change: instead of sonicator for cell lysis, French press was used for one of the samples

We've also made two samples of wt CCMV VLP ready for dialysis. There is only one modification applied in the protocol, instead of using the horrible sonicator, we used the French press, which is a great apparatus and easy to use. In the late afternoon we started with the dialysis.

Tuesday:

• Changed the buffer 3 times according to the dialysis protocol
• Made an appointment for DLS

written by: Mark