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From 2012.igem.org

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Greenhouse Effect and the limitation of the fossil fuels have always been a concern to people on Earth. So our team, NCTU_Formosa, hopes to produce isobutanol as a biofuel by bacteria. This concept will be achieved by E.coli containing plasmids with a lactose promoter, 5 RBSs, 4 zinc fingers, and 4 proteinic genes. With the temperature regulating system, we successfully prevent toxic intermediate interfering isobutanol production. This biofuel factory can provide more affordable and higher quantity biofuel, furthermore lower the burden of our Earth.