Team:TU Munich/Results/Biobricks

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Revision as of 12:16, 23 October 2012 by Fabian Froehlich (Talk | contribs)

Contents

BioBricks

BioBricks constructed during iGEM 2012

Overview for TU Munich's iGEM project 2012
BioBicks sorted according to our different subprojects
light inducible (A) ethanol inducible (B) constitutive (C) limonene (D) caffeine (E) xanthohumol (F) thaumatin (H)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801030 BBa_K801030] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801020 BBa_K801020] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801000 BBa_K801000] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801060 BBa_K801060] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801070 BBa_K801070] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801090 BBa_K801090] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801080 BBa_K801080]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801031 BBa_K801031] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801001 BBa_K801001] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801061 BBa_K801061] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801071 BBa_K801071] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801091 BBa_K801091] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801081 BBa_K801081]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801032 BBa_K801032] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801002 BBa_K801002] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801062 BBa_K801062] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801072 BBa_K801072] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801092 BBa_K801092]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801033 BBa_K801033] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801003 BBa_K801003] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801063 BBa_K801063] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801073 BBa_K801073] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801093 BBa_K801093]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801034 BBa_K801034] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801004 BBa_K801004] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801064 BBa_K801064] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801074 BBa_K801074] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801094 BBa_K801094]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801035 BBa_K801035] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801010 BBa_K801010] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801065 BBa_K801065] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801075 BBa_K801075] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801095 BBa_K801095]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801036 BBa_K801036] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801011 BBa_K801011] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801066 BBa_K801066] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801076 BBa_K801076] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801096 BBa_K801096]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801037 BBa_K801037] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801012 BBa_K801012] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801077 BBa_K801077] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801097 BBa_K801097]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801038 BBa_K801038] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801098 BBa_K801098]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801039 BBa_K801039]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801040 BBa_K801040]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801041 BBa_K801041]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801043 BBa_K801043]

List of all Parts

<groupparts>iGEM012 TU_Munich</groupparts>

Experiences with existing BioBricks

Rating working BioBricks

[http://partsregistry.org/Part:BBa_K300007:Experience Yeast Integration Vector BBa_K300007] (Partly showed functionality)

Identification of incorrect BioBricks

[http://partsregistry.org/Part:BBa_K268000:Experience#User_Reviews| Forbidden restriction sites in pSB6A0 (BBa_K268000g)]

Improvement of an existing BioBrick: [http://partsregistry.org/Part:BBa_K801100 BBa_K801100]

In 2012 the [http://partsregistry.org Registry of Standard Biological Parts] defined <partinfo>BBa_J04450</partinfo> as the standard shipping part that is required for submission of backbones, creating the need for a RFC25 compatible standard shipping part.

We extended the standard compability of the RFP coding device (<partinfo>BBa_J04450</partinfo>) to RFC10 and [http://hdl.handle.net/1721.1/45140 RFC25] by adding the NgoMIV and AgeI restriction sites into the prefix and suffix of this part. Additionally two AgeI restriction sites that were present in the generator itself were deleted.

This part may be used as a standard insert for RFC10 as well as [http://hdl.handle.net/1721.1/45140 RFC25] backbones. This improvement became necessary because insertion of <partinfo>BBa_J04450</partinfo> into a RFC25 compatible backbone leads to the deletion of the desired [http://hdl.handle.net/1721.1/45140 RFC25] restriction sites that are needed for protein fusions.

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