From 2012.igem.org

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• Official Team Profile
• Human Practices
• Caffeinated coli
• ZombiE.coli
• Spinach reporter
• Notebook
• Parts Submitted
• Safety
• Attributions

Team University of Texas at Austin

The University of Texas is the flagship public university of the UT system and resides in Austin, Texas. It's a fun place to live and a great place to do science.

Who we are

Advisor: Prof. Jeff Barrick Grad students: Jared Ellefson Michael Hammerling Erik Quandt Undergrads: Razan Alnahhas Logan Bachman Will Darden Aurko Dasgupta Peter Otoupal Ben Slater

Jeff Barrick; Assistant Professor of Chemistry and Biochemistry Jared Ellefson; Man with the Plan and part time Blues Brother Michael Hammerling; second year Graduate student in Cell and Molecular Biology Erik Quandt; Graduate student in Cell and Molecular Biology Razan Alnahhas: third year undergraduate studying human biology Logan Bachman; third year undergraduate biochemistry major, also awesome Will Darden: third year undergraduate studying Computational Biology Aurko Dasgupta: fourth year Cell and Molecular Biology student Peter Otoupal ; fourth year Chemical Engineering undergraduate, with a penchant for debauchery Ben Slater: Undergrad in junior year; studying cellular and molecular biology

Attributions

Advisors

• Prof. Jeff Barrick - faculty sponsor. A little graphic design for the website and coordinated getting Pseudomonas strain for Caffeinated coli. Found numbers to do calculation of number of guanines in E. coli.
• Jared Ellefson - Main ZombiE.coli project advisor.
• Michael Hammerling - Main Spinach-mCherry dual reporter project advisor. Advised students on lab work for ZombiE.coli and Caffeinated coli.
• Erik Quandt - Main caffeinated coli project advisor.

Team

• Razan Alnahhas -Constructed and tested pConverter.
• Logan Bachman - Constructed and tested Spinach and Spinach-mCherry reporters.
• Will Darden - Performed simulations of ZombiE.coli. Analysis of whole-genome shotgun sequencing of Pseudomonas putida CBB5.
• Aurko Dasgupta - Human practices, safety officer.
• Peter Otoupal - Assayed Caffeinated Coli constructs built by Ben. Performed caffeinated beverage and caffeine growth tests for decaffeination operon.
• Ben Slater - Constructed and tested pReporter and pReporter.permaflipped. Designed website's CSS stylesheet, navbar, and header. Assisted Peter and Erik with Caffeinated coli assays and construction.

Others

• [http://www.engineering.uiowa.edu/cbe/faculty-staff/mani-v-subramanian Prof. Mani Subramanian and Ryan Summers of the University of Iowa] for generously sharing the Pseudomonas putida CBB5 strain with us.
• [http://openwetware.org/wiki/User:Xi_Che Xi Chen of the Ellington Lab] for the original pET plasmid containing the spinach aptamer.
• [http://openwetware.org/wiki/CH391L/S12 Colleagues in the Spring 2012 CH 391L Synthetic Biology class] at UT Austin for ideas and research that inspired these projects.