Team:Fatih-Medical/Sherlocoli/Results
From 2012.igem.org
![](https://static.igem.org/mediawiki/2012/archive/f/f7/20120926021353!Sherlocoli_Overview_%281%29.png)
![](https://static.igem.org/mediawiki/2012/archive/f/f7/20120926235712!Sherlocoli_Overview_%281%29.png)
• EpCAM binding docks are cloned and confirmed. The gene sequences of EpCAM binding docks are synthesized particularly and separately. [See details] The confirmation electrophoresis and PCR afterwards were found correct. [See Imagexx] BBa_K772001 and BBa_K772002 are fixed together via 3A assembly method. After transformation into E.coli, the gel bands match with their original length; therefore we proceeded to the next step: Sequencing.
![](https://static.igem.org/mediawiki/igem.org/archive/e/e0/20120927030036!20120925_161036.jpg)
![](https://static.igem.org/mediawiki/igem.org/archive/e/e0/20120927030055!20120925_161036.jpg)
• Binding docks are sequenced and found correct. Sequence results overlap with the original sequences and show that these two parts are assembled correctly and ready to test for the synthesis and its function.
![](https://static.igem.org/mediawiki/2012/c/c5/Sekans.png)
![](https://static.igem.org/mediawiki/2012/archive/f/f7/20120927035038!Sherlocoli_Overview_%281%29.png)
![](https://static.igem.org/mediawiki/igem.org/archive/e/e0/20120927030300!20120925_161036.jpg)