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Team:TU-Eindhoven/LEC/Lab
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Revision as of 15:10, 26 September 2012 by Nickveeken (Talk | contribs)
Expected very soon!
Plasmid construction
The following plasmids have been constructed:
- pYES2/R-GECO, pYES2/G-GECO , pYES2/B-GECO
- pYES3/R-GECO, pYES3/G-GECO , pYES3/B-GECO
- pET28a/R-GECO, pET28a/G-GECO , pET28a/B-GECO
- pSB1C3/R-GECO, pSB1C3/G-GECO, pSB1C3/B-GECO
The following plasmids were received from addgene.org:
- CMV/R-GECO, CMV/G-GECO , CMV/B-GECO
These plasmids were kindly donated by H. Iida & K. Iida:
- pBCT/CCH1H, pBCT/CCH1H-HA4, pBCT/CCH1H-EGFP, YCpT-MID1, YCpT-MID1-EGFP
GECO protein expression and isolation
The three GECOs have been expressed in E. coli BL21 to yield a large amount of the proteins for characterization. Cells were cultured in large Erlenmeyer flasks. The results were visually impressive: After lysis the flasks turned vibrantly red and green respectively. The blue GECO however looked much the same as the green GECO.
TODO: COLUMN FILTRATION
