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Team:Peking/Modeling/Luminesensor/Simulation
From 2012.igem.org
ODE model
According to the previous network and ODE model, we listed all the differential equations and simulated this system with MATLAB with equations listed as
And parameters as
| Parameter | Value | Unit | Description | Source |
| k1 | 3.x10-4 | s-1 | vivid decay rate constant | |
| k2 | 5.6x10-5 | s-1 | vivid dissociation rate constant | [3] |
| k3 | 8.x10-4 | s-1 | monomer LexA releasing rate constant from specific binding site | |
| k4 | 1.x10-3 | s-1 | binded monomer LexA dissociation rate constant | |
| k5 | 1.x10-4 | s-1 | dimered LexA releasing rate constant from specific binding site | |
| K1(Dark) | 0 | 1 | equilibrium excitation constant on dark | |
| K1(Light) | 1.x10+3 | 1 | equilibrium excitation constant on light | |
| K2 | 7.7x10-5 | (n mol/L)-1 | vivid association equilibrium constant | [1] |
| K3 | 1.x10-3 | (n mol/L)-1 | monomer LexA binding equilibrium constant with specific binding site | [2] |
| K4 | K2xK5/K3 | (n mol/L)-1 | binded monomer LexA association equilibrium constant | Thermal Principle |
| K5 | 1. | (n mol/L)-1 | dimered LexA binding equilibrium constant | [2] |
| [LG]0 | 1000 | n mol/L | initial concentration of Luminesensor in ground state | |
| [LA]0 | 0 | n mol/L | initial concentration of Luminesensor in active state | |
| [LA2]0 | 0 | n mol/L | initial concentration of dimered Luminesensor | |
| [DL]0 | 100 | n mol/L | initial concentration of free specific binding site on DNA | high-copy plasmid |
| [LGDL]0 | 0 | n mol/L | initial concentration of dimered Luminesensor binded Luminesensor in ground state | |
| [LADL]0 | 0 | n mol/L | initial concentration of dimered Luminesensor binded Luminesensor in active state | |
| [LA2DL]0 | 0 | n mol/L | initial concentration of binded and dimered Luminesensor |
We specifically watched three expressions to understand the mechanism for Luminesensor.
| Expression | Description | Remark |
| rb = 1 - [DL]/[DT] | This indicates the repressing degree. | DT indicates the total specific binding sites, while the DL indicates the free ones among DT. |
| rd = 2[LA2X]/[LT] | This indicates the dimerizing degree. | LT indicates the total Luminesensor molecules, and LA2X indicates all dimered Luminesensor molecules, i.e. LA2 + LA2DL. |
| ra = ( [LAX] + 2[LA2X] ) /[LT] |
This indicates the activating degree. | LAX indicates all monomer Luminesensor molecules, i.e. LA + LADL. |
The simulation result is shown below:
Figure 2. ODE Simulation Result of the prototype Luminesensor.
From the figures above, we discovered that the activation and decay of Luminesensor are the key points of progress, and the activating rate is the most sensitive to light intensity. The promoter will be repressed even though the Luminesensor has not all dimerized.
Stochastic Simulation
In order to verify the robustness of Luminesensor function, we simulated this reaction network with a stochastic model. By estimating the volume of a cell, we converted the concentration of a component into the number of molecules by 1 n mol/L : 1. The result are shown below:
Figure 3. Stochastic Simulation Result of the prototype Luminesensor.
According to the figure above, the noise did not influence this system. Thus, the Luminesensor is expected to work theoretically. Besides, the average value of stochastic simulation is coupled with the result of ODE model, which in turn proves the self-consistency of our ODE model.
Simulation for GFP expression
regulated by Luminesensor
In order to see whether our model is effective to present the downstream gene expression under the control of Luminesensor, we added transcription and translation process after the modeling of DNA binding process. In addition, we considered the delay of translation initiation time and the growth of cell. The simulation below represents the GFP expression of the cell regulated by Luminesensor. After a long time in the light condition, where the GFP expression is inhibited, from t=0h, the cells are moved into dark and begin to express GFP. The GFP expression level according to time is recorded.
Figure 4. ODE Simulation Result is correspond to the experiment data of GFP expression level according to time from, which suggests that our model is effective to present the experiment situation.
Reference
- 1. Zoltowski, B.D., Crane, B.R.(2008). Light Activation of the LOV Protein Vivid Generates a Rapidly Exchanging Dimer. Biochemistry, 47: 7012: 7019
- 2. Mohana-Borges, R., Pacheco, A.B., Sousa, F.J., Foguel, D., Almeida, D.F., and Silva, J.L. (2000). LexA repressor forms stable dimers in solution. The role of specific DNA in tightening protein-protein interactions. J. Biol. Chem., 275: 4708: 4712
- 3. Zoltowski, B.D., Vaccaro, B., and Crane, B.R. (2009). Mechanism-based tuning of a LOV domain photoreceptor. Nat. Chem. Biol. 5: 827: 834
