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Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box | Label | Name | Part | Plasmid | Source | Sequence | Added by |
|---|---|---|---|---|---|---|---|
| Plasmid Box 1 | cadA | ASKA cadA plasmid | Barrick Lab | ||||
| Plasmid Box 1 | ldcC | ASKA ldcC plasmid | Barrick Lab | ||||
| Plasmid Box 1 | pIGM001 | LasR Promoter | [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | pSB1A2 | 2012 kit plate 1 (12A) | Verified | Razan |
| Plasmid Box 1 | pIGM002 | LasR | [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] | pSB3K3 | 2012 kit plate 1 (14J) | Verified | Razan |
| Plasmid Box 1 | pIGM003 | Lac Promoter | [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | pSB1A2 | 2012 kit plate 1 (1D) | Razan | |
| Plasmid Box 1 | pIGM004 | GFP | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | pSB3K3 | 2012 kit plate 2 (15L) | Verified | Ben |
| Plasmid Box 1 | pIGM005 | RFP (use IGM021 instead) | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | pSB1AK3 | 2012 kit plate 3 (12O) | Verified | Ben |
| Plasmid Box 1 | pIGM006 | LasI | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | pSB1A2 | 2012 kit plate 2 (10N) | Verified | Ben |
| Plasmid Box 1 | pIGM007 | RBS + LacI | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | pSB1A2 | 2012 kit plate 1 (7L) | Aurko | |
| Plasmid Box 1 | pIGM008 | Double terminator (BBa_B0010 - BBa_B0012) | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | pSB1AK3 | 2012 kit plate 1 (23L) | Aurko | |
| Plasmid Box 1 | pIGM009 | J23100 promoter - sfGFP (Part:BBa_K515105) | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] | pSB1C3 | 2012 kit plate 5 (1K) | ||
| Plasmid Box 1 | pIGM014 | Plasmid pSB1A3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1A3 | 2012 kit plate 1 (1G) | Logan | |
| Plasmid Box 1 | pIGM015 | Plasmid pSB1C3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1C3 | 2012 kit plate 1 (3A) | Logan | |
| Plasmid Box 1 | pIGM016 | Plasmid pSB1K3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1K3 | 2012 kit plate 1 (5A) | Logan | |
| Plasmid Box 1 | pIGM017 | Plasmid pSB1T3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1T3 | 2012 kit plate 1 (7A) | Logan | |
| Plasmid Box 1 | pIGM021 | RFP | [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] | pSB1A2 | 2012 kit plate 1 (13F) | Incorrect | Ben |
| Plasmid Box 1 | pIGM022 | pAK.TAQ | Jared | Mike | |||
| Plasmid Box 1 | pIGM023 | pET21.KOD | Jared | Mike | |||
| Plasmid Box 1 | pIGM024 | pQL269 | Jared | Mike | |||
| Plasmid Box 1 | pIGM025 | pACYC.T7.GFP | Jared | Mike | |||
| Plasmid Box 1 | pIGM026 | sfGFP | pET21 | Razan |
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box | Slot | Label | Description | Strain | Source | Added by |
|---|---|---|---|---|---|---|
| iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab | ||
| iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab | ||
| iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab | ||
| iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab | ||
| iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab | ||
| iGEM 2012 Box 1 | 7 | IGM009 | crdASC (4864 bp) | TOP10 | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 8 | IGM010 | crdASC (4943 bp) | TOP10 | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 9 | IGM011 | crdASC (4864 bp) | BL21(DE3) | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 10 | IGM012 | crdASC (4943 bp) | BL21(DE3) | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 11 | IGM013 | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) | epi300 | Parts Kit | |
| iGEM 2012 Box 1 | IGM014 | pSB1A3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
| iGEM 2012 Box 1 | IGM015 | pSB1C3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
| iGEM 2012 Box 1 | IGM016 | pSB1K3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
| iGEM 2012 Box 1 | IGM017 | pSB1T3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
| iGEM 2012 Box 1 | IGM018 | pSB1A2 with LasR Promoter Bba_R0079 | TOP10 | Parts Kit | Razan | |
| iGEM 2012 Box 1 | IGM019 | pSB2K3 with LasR Bba_C0079 | TOP10 | Parts Kit | Razan | |
| iGEM 2012 Box 1 | IGM020 | pSB1A2 with Lac Promoter Bba_R0010 | TOP10 | Parts Kit | Razan | |
| iGEM 2012 Box 1 | IGM021 | J23151_GFP_pSB3K3: Bba_I20270 | TOP10 | Parts Kit | Erik | |
| iGEM 2012 Box 1 | IGM022 | J23101_GFP_pSB3K3: Bba_I20260 | TOP10 | Parts Kit | Erik | |
| iGEM 2012 Box 1 | IGM023 | pet21.KOD | TOP10 | Jared | ||
| iGEM 2012 Box 1 | IGM024 | pQL269 | TOP10 | Jared | ||
| iGEM 2012 Box 1 | IGM025 | pACYC.T7.GFP | TOP10 | Jared | ||
| iGEM 2012 Box 1 | IGM026 | pSB1A2-J06504 (RFP--incorrect sequence) | TOP10 | Parts Kit | Ben | |
| iGEM 2012 Box 1 | IGM027 | pAK.TAQ | TOP10 | Jared |
ZombiE.coli parts
| Plasmid | Part | Description | Source |
|---|---|---|---|
| pReporter | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) | 2012 kit plate 2 (15L) |
| pReporter | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | RFP protein generator - PoPS->RFP | 2012 kit plate 3 (12O) |
| pReporter | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | LasI protein generator (TERM-) | 2012 kit plate 2 (10N) |
| pReporter | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | RBS + LacI | 2012 kit plate 1 (7L) |
| pReporter | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | Double terminator (BBa_B0010-BBa_B0012) | 2012 kit plate 1 (23L) |
| pConverter | [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | LasR Promoter | 2012 kit plate 1 (12A) |
| pConverter | [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] | LasR | 2012 kit plate 1 (14J) |
| pConverter | [http://partsregistry.org/Part:BBa_K082016 BBa_K082016] | LacIM1 | Parts Registry |
| pConverter | [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | LacI Promoter | 2012 kit plate 1 (1D) |
| pConverter | CRE | pQL269 Plasmid |
