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From 2012.igem.org

Attributions

Many thanks to all those who helped us, advised us and encouraged us over the summer !

The team

The team is composed of a mix of students, from L2 (2nd year after baccalaureate) to M2 (5th year after baccalaureate). We worked together from mid june to the end of september, some full time, some part time. More details can be found on the team page.

Cloning

All cloning work was done by the team in iSSB, Evry. Supervisor Thomas Landrain gave advice on cloning stategy throughout the project.

Animal work

Adult frogs are kept in the animal house shared between the Metamorphosys team of iSSB and Watchfrog (a company). The adult frogs were handled by Nicolas Pollet's team (for HCG injection, egg and sperm recovery). For the auxin toxicity tests, auxin penetration experiments and the E. coli to Xenopus communication devices, the fertilised eggs were provided by the metamorphosys team. For all tests of biobricks in Xenopus , the team was provided with unfertilised eggs and sperm, and in vitro fertilisation and DNA injection were done by team members under the supervision of Nicolas Pollet. Eggs were sorted and kept and analysed by the team, in the Metamorphosys labs.

Microscopy

Most microscopy photos were taken in the Metamorphosys labs by the team, with the help and supervision of Nicolas Pollet, Aurore Thélie and Léna Vouillot (Postdoc and PhD student in Metamorphosys team). We would like to thank Dr. Daniel Stockholm and Genethon for letting us use LSM 510 META Laser Scanning Microscope from Zeiss, which was used with his help and that of Nicolas Pollet and Aurore Thélie for high definition fluorescence images.

HPLC

Many thanks to Damien Baud (Genoscope, Evry), who run our samples on HPLC, and to our advisor Anna who helped us analyse the spectra.