Team:TU-Eindhoven/Protocols

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Standard protocols and modifications

In the lab we used many standard protocols, however, some of were modified from literature. The reasons for modification are indicated briefly below.

BioBrick construction

Yeast transformation

The principal protocol for yeast transformation was Gietz and Shiestl (2007)^[1]

References

• [1]Gietz and Shiestl, ''High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method'', Nature Protocols (2007) vol. 2, issue 1, pp. 31-34