"

From 2012.igem.org

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Sponsors	Attributions

Overall project

We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down gluten. (1-2 paragraphs)

Project Details

-yeast

 assemble plasmids
   integrative - industrial
   centromeric - Ethan
   episomal - Guy
 His tag plasmids - Dave
  n-terminus
  c-terminus
 clone mating factor-alpha Steven

-bacteria

 assemble plasmids (3A) - Steven
 His tag plasmids - Dave
  n-terminus
  c-terminus
 secretion -Ethan

-Prolyl Endoprotease-A.niger Guy

 viable option?
 source?  cloning vs synthesize
   E.coli
   yeast

-functional protease assay

 fluorophore/quencher - Ryan
 mass spec - Ryan/Guy
 elisa - Ryan/Guy

-Modeling - Dave/Ryan

Results