Data page: Summary of what we have done this year (so far!)

New frog plasmids

Fig 3: a. Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.

K812000: PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system

One characterization image

One plasmid map

The auxin system

The auxin receiver system

Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.

List of the parts with links to the registry

Which ones are characterized and result in one sentence

The auxin emission system

Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.

List of the parts with links to the registry Which ones are characterized and result in one sentence

Goldenbricks

Summary

Fig 4: Summary of the GoldenBrick procedure

The GoldenBrick is a new assembly method for the partsregistry. If you want to know more, see this page.

Our favourites parts

K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control

The other parts we have created

K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
K812053: A GoldenBricked version of the strong RBS B0034
K812054: A GoldenBricked version of the RFP E1010
K812055: A GoldenBricked version of the terminator B0015
K812056: A GoldenBricked version of the pLac R0010 promoter
K812057: A GoldenBricked of an sfGFP protein
K812058: A GoldenBricked of medium strenght RBS J61107
K812059: A GoldenBricked of week RBS J61117

say we are testing them

Team:Evry/Data

From 2012.igem.org