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Team:TU Darmstadt/Materials/LB Tributyrin
From 2012.igem.org
LB-Agar-Tributyrin
About
Modification of LB plates for hydrolytic activity detection. Tributyrin serves as a model substrate and is a good indicator for hydrolytic activity.
Ingredients
- 5 g tryptone
- 10 g yeast extract
- 5 g NaCl
- 15 g agar-agar
- 15 mL Tributyrin medium
- 5 g gum arabic
- 5 mL Tributyrin
- ad. 15 mL dist. water
- sonification for 45 min.
- ad. 1 L dist. water
- autoclave for 20 min. at 121°C
- wait until the temperature decreased under 60°C and add the prefered antibiotic
- pour the medium into petri plates (1 L LB-Agar is equal to 40 plates)
- store the plates at 4°C
