Team:Tuebingen/NotebookAppendix

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Contents

Appendix

To complete this report we list all products and software used over the course of our project.

Constructs

<tdTadh1</td>

<tr><td>315</td><td>Pfet3</th><th>rox1</th><th>Tadh1</th></tr> <tr><td>315</td><td>Pfet3</th><th>mig1</th><th>Tadh1</th></tr> <tr><td>316</td><td>Panb1</th><th>lacZ</th><th>Tadh1</th></tr> <tr><td>316</td><td>Panb1</th><th>luciferase</th><th>Tadh1</th></tr> <tr><td>316</td><td>Psuc2</th><th>lacZ</th><th>Tadh1</th></tr> <tr><td>316</td><td>Psuc2</th><th>luciferase</th><th>Tadh1</th></tr> </table>

Protocols

Kits

  • Genaxxon Plasmid DNA Purification Mini Prep
  • Genaxxon Gel Extraction Mini Prep
  • Genaxxon PCR DNA Purification Mini Prep
  • Qiagen Plasmid Midi


Chemicals

We needed the following chemicals:

  • Ampicillin
    beta-lactam antibiotic
  • Agarose
    Polysaccharide, major component of Agar
  • Dimethylsulfoxid (DMSO)
    organic solvent
  • Acetic Acid
  • Ethylenediaminetetraacetic acid (EDTA)
  • Ethanol
  • TRIS
    buffer solution for enzymes
  • Nucleoside triphosphate
  • Trypton
  • Isopropanol
  • Isopropyl-β-D-thiogalactopyranosid (IPTG)
  • LB-medium
    used for growth of E.coli
  • Agar-Agar


Software

The following list of software was used in the team:

  • [http://ugene.unipro.ru/ Unipro UGENE]
    UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
  • [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial)
    All our primers were designed using Vector NTI which is also used by our advisors.
  • [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
    BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
  • [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]
  • [http://drive.google.com Google Drive]
    Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).
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