Team:Osaka/week8

September 16 (Sun)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Gel extraction :161,162
5. Ligation
   • 163(C):161(upstream)+151(downstream)+1-3A(vector)
   • 164(C):161(upstream)+152(downstream)+1-3A(vector)
   • 165(C):162(upstream)+151(downstream)+1-3A(vector)
   • 166(C):162(upstream)+152(downstream)+1-3A(vector)
6. Transformation of ligation products into DH5α E.coli
7. Transfer to liquid culture
8. Tolerance assay(140,150)

September 17 (Mon)

1. Transfer to liquid culture:163~166,

September 18 (Tue)

1. Miniprep of yesterday's culture:163~166
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Transformation of plasmid DNA into E.coli HST04 161~166

September 19 (Wed)

1. Transfer to liquid culture:161~166,

September 20 (Thu)

1. Preparation of glycerol stock of cell culture:161~166

September 21 (Fri)

September 22 (Sat)

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