Team:Osaka/week8

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Revision as of 04:44, 15 September 2012 by Toshi (Talk | contribs)


September 16 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Gel extraction :161,162
  5. Ligation
    • 163(C):161(upstream)+151(downstream)+1-3A(vector)
    • 164(C):161(upstream)+152(downstream)+1-3A(vector)
    • 165(C):162(upstream)+151(downstream)+1-3A(vector)
    • 166(C):162(upstream)+152(downstream)+1-3A(vector)
  6. Transformation of ligation products into DH5α E.coli
  7. Transfer to liquid culture

September 17 (Mon)

  1. Transfer to liquid culture:163~166,


September 18 (Tue)

  1. Miniprep of yesterday's culture:163~166
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transformation of plasmid DNA into E.coli: HST04 161~166

September 19 (Wed)

  1. Transfer to liquid culture:161~166,


September 20 (Thu)

  1. Preparation of glycerol stock of cell culture:161~166


September 21 (Fri)

September 22 (Sat)

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