Team:Osaka/week8
From 2012.igem.org
September 16 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction :161,162
- Ligation
- 163(C):161(upstream)+151(downstream)+1-3A(vector)
- 164(C):161(upstream)+152(downstream)+1-3A(vector)
- 165(C):162(upstream)+151(downstream)+1-3A(vector)
- 166(C):162(upstream)+152(downstream)+1-3A(vector)
- Transformation of ligation products
- To liquid culture