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From 2012.igem.org

Contents 1 Appendix 1.1 Kits and Protocols 1.2 Chemicals 1.3 Software

Appendix

To complete this report we list all products and software used over the course of our project.

Kits and Protocols

• Genaxxon Plasmid DNA Purification Mini Prep
• Genaxxon Gel Extraction Mini Prep
• Genaxxon PCR DNA Purification Mini Prep
• Qiagen Plasmid Midi

Chemicals

We needed the following chemicals:

• Ampicillin
  beta-lactam antibiotic
• Agarose
  Polysaccharide, major component of Agar
• Dimethylsulfoxid (DMSO)
  organic solvent
• Acetic Acid
• Ethylenediaminetetraacetic acid (EDTA)
• Ethanol
• TRIS
  buffer solution for enzymes
• Nucleoside triphosphate
• Trypton
• Isopropanol
• Isopropyl-β-D-thiogalactopyranosid (IPTG)
• LB-medium
  used for growth of E.coli
• Agar-Agar

Software

The following list of software was used in the team:

• [http://ugene.unipro.ru/ Unipro UGENE]
  UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.

• [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial)
  All our primers were designed using Vector NTI which is also used by our advisors.

• [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
  BLAST was our main sequence search tool. It was quite useful for controlling sequence results.

• [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]

• [http://drive.google.com Google Drive]
  Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).