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Team:SDU-Denmark/labwork/Protocols/PCR
From 2012.igem.org
| mRNA Isolation | PCR | Miniprep | Check Digest |
| Content | Content | Content | Content |
| Content | Content | Content | Content |
Polymerase Chain Reaction
Proof-reading PCR using Phusion Hot Start II
PCR program:
95°C for 30 seconds25-35 cycles: 98°C for 30 seconds Annealing temperature (5°C below primer melting temperature) 72°C for 15-30s/kb72°C for 5-10 minutes 4°C on hold
