Team:Wageningen UR/Journal/week11

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week 11: 9 july - 15 july

Office work

Lab work

TuYV

  • Two (non-readthrough) gene variants were digested and ligated into pSB1A3 backbone.


Written by: Wouter

PLRV

The start of the lab work on PLRV

We went out to the potato fields near Wageningen to look for PLRV infected potatoes. We thought we found some infected plants, based on the primary symptoms namely smaller sizes of the plants and curled leafs (figure 1).

Field.jpg

We contacted Kees Bus and Chris Cuperus, who are experts at the Wageningen University on potato diseases. Judging the pictures, they concluded that these plants were probably not infected by PLRV.

This week we started trial experiments for RNA isolation from potato plants.

Rt-pcr.jpg RNA isolate purity checked. RNA is of very good quality. Note that smaller rRNA bands visible in the leaf sample are derived from plant organelles such as plastids and chloroplasts


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