Team:TU Darmstadt/Materials/Ampicillin

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Contents

Ampicillin

About

Ampcillin is a beta-lactam antibiotic that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria.

Stock

  1. 4 g ampicillin (100 mg/mL)
  2. ad. 40 mL dist. water
  3. sterile filtration
  4. aliquot in 1 mL stocks and store at -20°C

Stability

  • Ampicillin is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
  • Culture plates can be stored for about 2 month at 4°C (as a batch in a bag or individually sealed with [http://en.wikipedia.org/wiki/Parafilm Parafilm])

References

  • http://en.wikipedia.org/wiki/Ampicillin
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