Team:TU Darmstadt/Labjournal/Transport
From 2012.igem.org
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===Week 1 (21.-25.05.12)=== | ===Week 1 (21.-25.05.12)=== | ||
- | * First [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ | + | * First [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Colony_PCR colony PCR] of ''Comamonas t.'' for isolation of the following genes: [https://2012.igem.org/Team:TU_Darmstadt/Protocols/tctA_505aa tctA 505aa] (1), [https://2012.igem.org/Team:TU_Darmstadt/Protocols/tctB_197aa tctB 197aa] (2), [https://2012.igem.org/Team:TU_Darmstadt/Protocols/tctA_503aa tctA_503aa] (3), [https://2012.igem.org/Team:TU_Darmstadt/Protocols/tctB_162aa tctB 162aa] (4), [https://2012.igem.org/Team:TU_Darmstadt/Protocols/tphC_322aa tphC 322aa] (5), 50µl reaction mixture each; analysis of [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] products by [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrohporesis Agarose gel electrohporesis] |
:https://static.igem.org/mediawiki/2012/1/15/W1_1_kolo_pcr.png | :https://static.igem.org/mediawiki/2012/1/15/W1_1_kolo_pcr.png |
Revision as of 12:45, 6 September 2012
Transport
Week 1 (21.-25.05.12)
- First colony PCR of Comamonas t. for isolation of the following genes: tctA 505aa (1), tctB 197aa (2), tctA_503aa (3), tctB 162aa (4), tphC 322aa (5), 50µl reaction mixture each; analysis of [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR] products by Agarose gel electrohporesis