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From 2012.igem.org

Revision as of 16:26, 5 September 2012 (view source) PierreYves (Talk | contribs) ← Older edit		Revision as of 16:26, 5 September 2012 (view source) PierreYves (Talk | contribs) Newer edit →
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	<h2>Monday 3rd</h2>		<h2>Monday 3rd</h2>
	<u><h5>Construction about a new pCS2+ with RFP insert</u></h5>		<u><h5>Construction about a new pCS2+ with RFP insert</u></h5>
		+
	-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI		-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI
		+
	-Purification gel to get only RFP insert ( gel image )		-Purification gel to get only RFP insert ( gel image )
		+
	-Extraction of the RFP insert		-Extraction of the RFP insert
		+
	-Ligation with pCS2+		-Ligation with pCS2+
		+
		+
		+
	<h2>Tuesday 3rd</h2>		<h2>Tuesday 3rd</h2>
		+
	<u><h5>Preparation of a new stock of electrocompetent cells T10</u></h5>		<u><h5>Preparation of a new stock of electrocompetent cells T10</u></h5>
		+
	<u><h5>Construction about a new pCS2+ with RFP insert :</u></h5>		<u><h5>Construction about a new pCS2+ with RFP insert :</u></h5>
	<i>Following</i>		<i>Following</i>
		+
	-Transformation by electroporation with T10 cells		-Transformation by electroporation with T10 cells
		+
	-Plates prepared		-Plates prepared

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Revision as of 16:26, 5 September 2012

Weeks:

June			July				August				September				October				November

Week 13: 3rd September - 9th September

Monday 3rd

Construction about a new pCS2+ with RFP insert

-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI -Purification gel to get only RFP insert ( gel image ) -Extraction of the RFP insert -Ligation with pCS2+

Tuesday 3rd

Preparation of a new stock of electrocompetent cells T10

Construction about a new pCS2+ with RFP insert :

Following -Transformation by electroporation with T10 cells -Plates prepared