Team:Evry/Notebook/w13

From 2012.igem.org

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<h1>Week 13: 3rd September - 9th September</h1>
<h1>Week 13: 3rd September - 9th September</h1>
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<h2>Monday 3rd</h2>
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'''[[
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Construction about a new pCS2+ with RFP insert :]]'''
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-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI
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-Purification gel to get only RFP insert ( gel image )
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-Extraction of the RFP insert
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-Ligation with pCS2+
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<h2>Tuesday 3rd</h2>
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'''[[
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'''[[Preparation of a new stock of electrocompetent cells T10]]'''
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Construction about a new pCS2+ with RFP insert :]]'''
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''Following''
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-Transformation by electroporation with T10 cells
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-Plates prepared
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</html>

Revision as of 16:22, 5 September 2012

Weeks:

June July August September October November

Week 13: 3rd September - 9th September

Monday 3rd

'''[[ Construction about a new pCS2+ with RFP insert :]]''' -Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI -Purification gel to get only RFP insert ( gel image ) -Extraction of the RFP insert -Ligation with pCS2+

Tuesday 3rd

'''[[ '''[[Preparation of a new stock of electrocompetent cells T10]]''' Construction about a new pCS2+ with RFP insert :]]''' ''Following'' -Transformation by electroporation with T10 cells -Plates prepared

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