Team:UNITN-Trento/Parts

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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
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      <h1><a href="/">Crust Away</a></h1>
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      <span class="logo-subtitle">iGEM Trento 2012</span>
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<h2>Our Parts</h2>
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<p>Here you can find all the Parts we built. Be sure to check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=UNITN-Trento">Team Parts Page on the Registry website</a>!</p>
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<table class="pgrouptable addborder" style="width: 925px;" cellpadding="0" cellspacing="0"><tbody><tr><th style="width: 7em;">Name</th><th colspan="">Type</th><th colspan="">Description</th><th colspan="">Designer</th><th class="blank_col">
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data">
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25292"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></form></th></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">BBa_K731000</a></td><td>Coding</td><td>CysE (Serine Acetyltransferase)</td><td >Jason Fontana, Daniele Rossetto, Giacomo Giacomelli</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25430"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">BBa_K731010</a></td><td>Coding</td><td>M256I CysE (Serine Acetyltransferase)</td><td >Jason Fontana, Daniele Rossetto, Giacomo Giacomelli</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25431"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">BBa_K731020</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating WT CysE</td><td >Jason Fontana</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25432"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">BBa_K731030</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating M256I CysE</td><td >Jason Fontana, Daniele Rossetto</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25433"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">BBa_K731040</a></td><td>Composite</td><td>Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</td><td >Jason Fontana, Daniele Rossetto</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25355"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">BBa_K731201</a></td><td>Regulatory</td><td>Inducible araC-pBAD promoter</td><td >Daniele Rossetto</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25356"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">BBa_K731250</a></td><td>Composite</td><td>Inducible araC-pBAD promoter with GFPmut3b, strong RBS</td><td >Daniele Rossetto</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25357"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">BBa_K731255</a></td><td>Composite</td><td>Inducible araC-pBAD promoter with GFPmut3b, weak RBS</td><td >Daniele Rossetto</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25439"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">BBa_K731300</a></td><td>Regulatory</td><td>LacI + LacIq promoter (reverse)</td><td >Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25440"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">BBa_K731400</a></td><td>Composite</td><td>IPTG inducible Cysteine desulfhydrase (CysDes)</td><td >Andrea Tassinari</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25444"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">BBa_K731480</a></td><td>Composite</td><td>IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</td><td >Andrea Tassinari, Francesco Guzzonato</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25446"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">BBa_K731500</a></td><td>Regulatory</td><td>Inducibile tac promoter and lac operon</td><td >Francesco Guzzonato</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25447"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">BBa_K731520</a></td><td>Composite</td><td>lactose-inducible sfGFP</td><td >Francesco Guzzonato</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25452"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731600">BBa_K731600</a></td><td>Coding</td><td>Cysteine Desulfhydrase (CysDes)</td><td >Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25409"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">BBa_K731700</a></td><td>Plasmid_Backbone</td><td>Low copy vector for terminators analysis with T7 RNApolymerase by fluorimetric assay</td><td >Giacomo Giacomelli, Anna Depetris</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25465"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731701">BBa_K731701</a></td><td>Measurement</td><td>BBa_K731700 with T7terminator (BBa_K731721)</td><td >Giacomo Giacomelli, Anna Depetris</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25466"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">BBa_K731702</a></td><td>Measurement</td><td>BBa_K731700 with BBa_K731722 terminator (BBa_B0010)</td><td >Giacomo Giacomelli, Anna Depetris</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25411"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">BBa_K731710</a></td><td>Plasmid_Backbone</td><td>Low copy vector for terminators analysis with Coli RNApolymerase by fluorimetric assay</td><td >Giacomo Giacomelli, Anna Depetris</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25464"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731711">BBa_K731711</a></td><td>Measurement</td><td>BBa_K731710 with T7terminator (BBa_K731721)</td><td >Giacomo Giacomelli, Anna Depetris</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25462"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">BBa_K731712</a></td><td>Measurement</td><td>BBa_K731710 with BBa_K731722 terminator (BBa_B0010)</td><td >Giacomo Giacomelli, Anna Depetris</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25413"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">BBa_K731721</a></td><td>Terminator</td><td>T7 terminator</td><td >Anna Depetris, Giacomo Giacomelli</td>
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<form method="post" action="/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&amp;group=UNITN-Trento" enctype="multipart/form-data"></form>
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<input type="hidden" name="Editing" value="Editing"><input type="hidden" name="part" value="25455"><input type="hidden" name="pgroup" value="iGEM2012"><input type="hidden" name="group" value="UNITN-Trento"></tr><tr><td><a class="noul_link" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">BBa_K731722</a></td><td>Terminator</td><td>B0010 Coli terminator</td><td >Anna Depetris, Giacomo Giacomelli</td>
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</tr></tbody></table>
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<div class="member">
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<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
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<div class=partDescription>
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<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">K731000 - CysE (Serine Acetyltransferase)</a></h2>
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<p>CysE is an enzyme involved in cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, a precursor for the biosynthesis of cysteine. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.</p>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731000">Registry Page</a>
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</div>
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</div>
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<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">K731010 - M256I CysE (Serine Acetyltransferase)</a></h2>
 +
<p>CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.
 +
M256I CysE shows enhanced secretion of cysteine and is not inhibited by Cysteine overproduction.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731010">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">K731020 - Inducible araC-pBAD promoter regulating WT CysE</a></h2>
 +
<p>The CysE gene (K731000) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731020">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">K731030 - Inducible araC-pBAD promoter regulating M256I CysE</a></h2>
 +
<p>The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731030">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">K731040 - Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE</a></h2>
 +
<p>A sfGFP tagged M256I CysE inducible by arabinose. This part was subcloned and characterized in pSB3C5.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731040">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">K731201 - Inducible araC-pBAD promoter</a></h2>
 +
<p style="font-size: 11px;">This E. coli promoter is positively regulated by arabinose.
 +
araC is the regulator protein: in absence of arabinose the protein binds to araI1 and araO2 and the DNA forms a loop that prevents RNA polymerase from binding the promoter. When arabinose is added to the culture it binds to araC, disabling the interaction with araI1 and araO2 sites, and also allowing it to bind araI site, promoting transcription.
 +
Promoter activity is also influenced by glucose levels: in its absence cAMP levels are high and the the complex cAMP+CAP (catabolite activator protein) binds to lacI promoting transcription. In presence of glucose, cAMP levels are low and promoter activity is inhibited.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731201">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">K731250 - Inducible araC-pBAD promoter with GFPmut3b, strong RBS</a></h2>
 +
<p>This part has been used for testing and characterization of araC-pBAD promoter (BBa_K731201). The part is composed of part BBa_K731201 and BBa_E0840.
 +
This part was successfully operated and characterized both in psB1C3 and psB3C5.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731250">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">K731255 - Inducible araC-pBAD promoter with GFPmut3b, weak RBS</a></h2>
 +
<p>This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200) when using a weak RBS. The part is composed of part BBa_K731200 and BBa_E0240.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731255">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">K731300 - LacI + LacIq promoter (reverse)</a></h2>
 +
<p>This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG, and it is a very useful device to control and regulate the expression of a gene of interest.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731300">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)</a></h2>
 +
<p>This part is composed of K731300 and K731600. It is an IPTG inducible device regulating the expression of a Cysteine Desulfhydrase from Treponema Denticola (CysDes). This enzyme is an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. The part has been successfully operated and characterized in psB1C3 and psB4K5.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731400">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">K731480 - IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)</a></h2>
 +
<p>A sfGFP tagged CysDes inducible by IPTG.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731480">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">K731500 - Inducibile tac promoter and lac operon</a></h2>
 +
<p>This is E.coli lactose-inducibile promoter.
 +
lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731500">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">K731520 - Lactose-inducible sfGFP</a></h2>
 +
<p>This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control.
 +
lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731520">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731600">K731600 - Cysteine Desulfhydrase (CysDes)</a></h2>
 +
<p>This part encode for a cysteine desulfhydrase from Treponema denticola, which is known to be a strong enzyme that produces H2S, ammonia and pyruvate starting from cysteine. It is often indicated also as Hly.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731600">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731700 - Low copy vector for terminators analysis with T7 RNApolymerase by fluorimetric assay</a></h2>
 +
<p>This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector is a modified pET21b; it contains Ampicillin resistance gene and is inducible by IPTG (T7promoter-lacO).
 +
The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">K731701 - BBa_K731700 with T7terminator (BBa_K731721)</a></h2>
 +
<p>T7 terminator T7 polymerase testing construct.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731700">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">K731702 - BBa_K731700 with BBa_K731722 terminator (BBa_B0010)</a></h2>
 +
<p>Coli terminator T7 polymerase testing construct.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731702">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">K731710 - Low copy vector for terminators analysis with Coli RNApolymerase by fluorimetric assay</a></h2>
 +
<p style="font-size: 11px;">This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector was obained by mutagenesis of BBa_K731700, replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (pTAC-lacO).
 +
The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731710">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731">K731711 - BBa_K731710 with T7terminator (BBa_K731721)</a></h2>
 +
<p>T7 terminator coli polymerase testing construct.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">K731712 - BBa_K731710 with BBa_K731722 terminator (BBa_B0010)</a></h2>
 +
<p>coli terminator coli polymerase testing construct 2.</p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731712">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">K731721 - T7 terminator</a></h2>
 +
<p>Gag ipsum dolar sit amet venenatis charlie sheen lose i'm watching u. Female weekend finals close enough derp top note so close architect yao. Face oboma unlock ipsum t-rex keyboard just simpson gag elephant bacon cuteness overload. </p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731721">Registry Page</a>
 +
</div>
 +
</div>
 +
 +
<div class="member bitleft">
 +
<img src="http://www.jasonfweb.com/igem2/img/team/Jason.jpg" alt="" />
 +
<div class=partDescription>
 +
<h2><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">K731722 - B0010 Coli terminator</a></h2>
 +
<p>Gag ipsum dolar sit amet venenatis charlie sheen lose i'm watching u. Female weekend finals close enough derp top note so close architect yao. Face oboma unlock ipsum t-rex keyboard just simpson gag elephant bacon cuteness overload. </p>
 +
<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K731722">Registry Page</a>
 +
</div>
 +
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Revision as of 14:14, 5 September 2012

Our Parts

Gallery

Our Parts

Here you can find all the Parts we built. Be sure to check out our Team Parts Page on the Registry website!

NameTypeDescriptionDesigner
BBa_K731000CodingCysE (Serine Acetyltransferase)Jason Fontana, Daniele Rossetto, Giacomo Giacomelli
BBa_K731010CodingM256I CysE (Serine Acetyltransferase)Jason Fontana, Daniele Rossetto, Giacomo Giacomelli
BBa_K731020CompositeInducible araC-pBAD promoter regulating WT CysEJason Fontana
BBa_K731030CompositeInducible araC-pBAD promoter regulating M256I CysEJason Fontana, Daniele Rossetto
BBa_K731040CompositeInducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysEJason Fontana, Daniele Rossetto
BBa_K731201RegulatoryInducible araC-pBAD promoterDaniele Rossetto
BBa_K731250CompositeInducible araC-pBAD promoter with GFPmut3b, strong RBSDaniele Rossetto
BBa_K731255CompositeInducible araC-pBAD promoter with GFPmut3b, weak RBSDaniele Rossetto
BBa_K731300RegulatoryLacI + LacIq promoter (reverse)Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli
BBa_K731400CompositeIPTG inducible Cysteine desulfhydrase (CysDes)Andrea Tassinari
BBa_K731480CompositeIPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)Andrea Tassinari, Francesco Guzzonato
BBa_K731500RegulatoryInducibile tac promoter and lac operonFrancesco Guzzonato
BBa_K731520Compositelactose-inducible sfGFPFrancesco Guzzonato
BBa_K731600CodingCysteine Desulfhydrase (CysDes)Andrea Tassinari, Daniele Rossetto, Giacomo Giacomelli
BBa_K731700Plasmid_BackboneLow copy vector for terminators analysis with T7 RNApolymerase by fluorimetric assayGiacomo Giacomelli, Anna Depetris
BBa_K731701MeasurementBBa_K731700 with T7terminator (BBa_K731721)Giacomo Giacomelli, Anna Depetris
BBa_K731702MeasurementBBa_K731700 with BBa_K731722 terminator (BBa_B0010)Giacomo Giacomelli, Anna Depetris
BBa_K731710Plasmid_BackboneLow copy vector for terminators analysis with Coli RNApolymerase by fluorimetric assayGiacomo Giacomelli, Anna Depetris
BBa_K731711MeasurementBBa_K731710 with T7terminator (BBa_K731721)Giacomo Giacomelli, Anna Depetris
BBa_K731712MeasurementBBa_K731710 with BBa_K731722 terminator (BBa_B0010)Giacomo Giacomelli, Anna Depetris
BBa_K731721TerminatorT7 terminatorAnna Depetris, Giacomo Giacomelli
BBa_K731722TerminatorB0010 Coli terminatorAnna Depetris, Giacomo Giacomelli

K731000 - CysE (Serine Acetyltransferase)

CysE is an enzyme involved in cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, a precursor for the biosynthesis of cysteine. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes.

Registry Page

K731010 - M256I CysE (Serine Acetyltransferase)

CysE is an enzyme involved In cysteine biosynthesis, also known as SAT. It catalyzes the acetylation of serine to give O-acetylserine, the CysE final precursor. Some O-acetylserine is also converted to N-acetylserine, which in turn triggers the assimilation of sulfate through specific genes. M256I CysE shows enhanced secretion of cysteine and is not inhibited by Cysteine overproduction.

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K731020 - Inducible araC-pBAD promoter regulating WT CysE

The CysE gene (K731000) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.

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K731030 - Inducible araC-pBAD promoter regulating M256I CysE

The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.

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K731040 - Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE

A sfGFP tagged M256I CysE inducible by arabinose. This part was subcloned and characterized in pSB3C5.

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K731201 - Inducible araC-pBAD promoter

This E. coli promoter is positively regulated by arabinose. araC is the regulator protein: in absence of arabinose the protein binds to araI1 and araO2 and the DNA forms a loop that prevents RNA polymerase from binding the promoter. When arabinose is added to the culture it binds to araC, disabling the interaction with araI1 and araO2 sites, and also allowing it to bind araI site, promoting transcription. Promoter activity is also influenced by glucose levels: in its absence cAMP levels are high and the the complex cAMP+CAP (catabolite activator protein) binds to lacI promoting transcription. In presence of glucose, cAMP levels are low and promoter activity is inhibited.

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K731250 - Inducible araC-pBAD promoter with GFPmut3b, strong RBS

This part has been used for testing and characterization of araC-pBAD promoter (BBa_K731201). The part is composed of part BBa_K731201 and BBa_E0840. This part was successfully operated and characterized both in psB1C3 and psB3C5.

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K731255 - Inducible araC-pBAD promoter with GFPmut3b, weak RBS

This part has been used for testing and characterization of araC-pBAD promoter (part BBa_K731200) when using a weak RBS. The part is composed of part BBa_K731200 and BBa_E0240.

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K731300 - LacI + LacIq promoter (reverse)

This transcriptional regulator includes the LacI repressor and its constitutive promoter LacIq which operates in the reverse direction. The LacI repressor protein is inhibited by IPTG, and it is a very useful device to control and regulate the expression of a gene of interest.

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K731400 - IPTG inducible Cysteine desulfhydrase (CysDes)

This part is composed of K731300 and K731600. It is an IPTG inducible device regulating the expression of a Cysteine Desulfhydrase from Treponema Denticola (CysDes). This enzyme is an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. The part has been successfully operated and characterized in psB1C3 and psB4K5.

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K731480 - IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)

A sfGFP tagged CysDes inducible by IPTG.

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K731500 - Inducibile tac promoter and lac operon

This is E.coli lactose-inducibile promoter. lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow.

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K731520 - Lactose-inducible sfGFP

This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control. lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.

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K731600 - Cysteine Desulfhydrase (CysDes)

This part encode for a cysteine desulfhydrase from Treponema denticola, which is known to be a strong enzyme that produces H2S, ammonia and pyruvate starting from cysteine. It is often indicated also as Hly.

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K731700 - Low copy vector for terminators analysis with T7 RNApolymerase by fluorimetric assay

This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector is a modified pET21b; it contains Ampicillin resistance gene and is inducible by IPTG (T7promoter-lacO). The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.

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K731701 - BBa_K731700 with T7terminator (BBa_K731721)

T7 terminator T7 polymerase testing construct.

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K731702 - BBa_K731700 with BBa_K731722 terminator (BBa_B0010)

Coli terminator T7 polymerase testing construct.

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K731710 - Low copy vector for terminators analysis with Coli RNApolymerase by fluorimetric assay

This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector was obained by mutagenesis of BBa_K731700, replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (pTAC-lacO). The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.

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K731711 - BBa_K731710 with T7terminator (BBa_K731721)

T7 terminator coli polymerase testing construct.

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K731712 - BBa_K731710 with BBa_K731722 terminator (BBa_B0010)

coli terminator coli polymerase testing construct 2.

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K731721 - T7 terminator

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K731722 - B0010 Coli terminator

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Retrieved from "http://2012.igem.org/Team:UNITN-Trento/Parts"