Team:Cambridge/Lab book/Week 10
From 2012.igem.org
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===Friday (31/08/12)=== | ===Friday (31/08/12)=== | ||
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+ | '''[[Team:Cambridge/Protocols/PCRProtocol|PCR of PJS130 vector for MgRS construct]]''' | ||
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+ | [[File:PJS130gel1.jpg|250px|right|thumb|Gel from amplification of PJS130 fragments. Lanes 2 + 3: Fragment A. Lanes 4 + 5: Fragment B with eight codon substitution. Lanes 6 + 7: Fragment B without eight codon substitution. Lane 8: Negative control.]] | ||
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+ | *Cycle settings: | ||
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+ | :*Melting - 98 °C - 10 seconds | ||
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+ | :*Annealing - 58 °C - 30 seconds | ||
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+ | :*Elongation - 72 °C - 120 seconds | ||
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+ | *Products of correct sizes produced for all reactions, although lanes 2 and 4 failed to produce any product, despite primer smear. Most likely, template was not added, or one of the primers was not added. | ||
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+ | *Products excised and purified. | ||
===Saturday (01/09/12)=== | ===Saturday (01/09/12)=== |
Revision as of 17:03, 31 August 2012
Week: | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
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Contents |
Monday (27/08/12)
Tuesday (28/08/12)
Wednesday (29/08/12)
Thursday (30/08/12)
PCR of Mg2+ riboswitch from genomic DNA
- Cycle settings:
- Melting - 98 °C - 10 seconds
- Annealing - 60 °C - 30 seconds
- Elongation - 72 °C - 110 seconds
- Fragments of correct size produced for all except lane 5 produced. In this lane, DNA appears to have accumulated in the well, indicating it may be genomic. In future, will use lower numbers of template cells to avoid getting so much genomic DNA.
- Products extracted and purified.
Friday (31/08/12)
PCR of PJS130 vector for MgRS construct
- Cycle settings:
- Melting - 98 °C - 10 seconds
- Annealing - 58 °C - 30 seconds
- Elongation - 72 °C - 120 seconds
- Products of correct sizes produced for all reactions, although lanes 2 and 4 failed to produce any product, despite primer smear. Most likely, template was not added, or one of the primers was not added.
- Products excised and purified.
Saturday (01/09/12)
Sunday (02/09/12)