"
Team:Osaka/Protocols
From 2012.igem.org
(Difference between revisions)
(Created page with "{{Osaka}} __NOTOC__ == Protocols == === Cell survival assay 1: UV irradiation === #Pre-culture transformed cells in 3ml of LB medium at 37°C for 16h. #Induce parts with IPTG...") |
|||
| Line 1: | Line 1: | ||
| - | {{ | + | {{Osaka2}} |
__NOTOC__ | __NOTOC__ | ||
Revision as of 02:21, 28 August 2012
Protocols
Cell survival assay 1: UV irradiation
- Pre-culture transformed cells in 3ml of LB medium at 37°C for 16h.
- Induce parts with IPTG addition (to final concentration of 100µM) for 1h.
- Dilute pre-culture samples according to intended UV energy dosage (if dosage is high, lower dilution rate is needed as fewer cells are expected to survive).
- Transfer 50μl of diluted pre-culture onto LB agar plates and spread evenly. Air-dry the plates to remove excess wetness.
- Irradiate cells on the agar with UV light at desired energy dosage.
- Wrap plates in aluminium foil and incubate at 37°C.
- After 16h, count number of colonies formed on control (non-irradiated) and UV-irradiated plates.
