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Team:Evry/Notebook/w11
From 2012.igem.org
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<h3>pSC2+ RFP, pSC2+ IaaH</h3> | <h3>pSC2+ RFP, pSC2+ IaaH</h3> | ||
Transformation results: 2 colonies pink on pSC2+ RFP plate, no one on the pSC2+ IaaH plate | Transformation results: 2 colonies pink on pSC2+ RFP plate, no one on the pSC2+ IaaH plate | ||
| - | PCR colony on the 2 pink colonies pSC2+ RFP with P26 and P27 primers | + | PCR colony on the 2 pink colonies pSC2+ RFP with P26 and P27 primers <br> |
| + | |||
| + | <h3>IaaH-IaaM-IRES</h3> | ||
| + | After the PCR of IaaH, IaaM and IRES (16th August) digestion with EcoRI and PstI (but also or pSB1C3) then gel extraction<br> | ||
| + | Ligation of Ires, IaaH and IaaM in pSB1C3<br> | ||
| + | <h2>Tuesday, 21st August</h2> | ||
| + | Transformation of pSB1C3-IRES, pSB1C3-IaaH and pSB1C3-IaaM plus control ligation pSB1C3 without insert<br> | ||
| + | |||
| + | Injection pNHK60 (50ng/microL) into xenopus tropicalis eggs<br> | ||
| + | |||
</html> | </html> | ||
Revision as of 16:46, 21 August 2012
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 11: 20th August - 26th August
Monday, 20th August
pSC2+ RFP, pSC2+ IaaH
Transformation results: 2 colonies pink on pSC2+ RFP plate, no one on the pSC2+ IaaH plate PCR colony on the 2 pink colonies pSC2+ RFP with P26 and P27 primersIaaH-IaaM-IRES
After the PCR of IaaH, IaaM and IRES (16th August) digestion with EcoRI and PstI (but also or pSB1C3) then gel extractionLigation of Ires, IaaH and IaaM in pSB1C3
Tuesday, 21st August
Transformation of pSB1C3-IRES, pSB1C3-IaaH and pSB1C3-IaaM plus control ligation pSB1C3 without insertInjection pNHK60 (50ng/microL) into xenopus tropicalis eggs
