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Team:Wageningen UR/Journal/week2
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pellet stored at -20 degree Celcius, 50 ml greiner tubes | pellet stored at -20 degree Celcius, 50 ml greiner tubes | ||
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| + | Miniprep following the Gene Jet Fermentas protocol | ||
| + | *pet28 | ||
| + | *standard 10 stock 1 | ||
| + | *standard 10 stock 7 | ||
Revision as of 07:44, 31 May 2012
week 2: 7 may - 11 may
office work
lab work
7 may
E. coli in liquid LB
3 plates per strain were picked and grown in 25 ml LB. all plates grown well accept for amp plates, due to drying effects.
8 may
10:00 Continue growth. All 3 strains in shaker, 180 RPM, 30 degree celcius.
4 x 1 L SOB is made
16:55 culture mach1 and DH5X are plated 10x and 1000x diluted on LB plates. Dilutions are done with demi water.
Plates were placed in 37 degree Celcius stove
9 may
9:15 10x dilution was to low, overgrown 1000x was nearly overrown, but still acceptable.
picked 1 MACH1 and 1 DH5X colony and grown in 10 ml SOB medium, 37 degree Celcius, 180 RPM
10 may
MACH1 competent cells followin pinky's protocol. with the following diviations
- 4x 500 ml SOB
- SLA 3000 rotor
OD harvest at: 0.5 -0.45
11 may
CCMV protocol inoculated at 8:45 IPTG at 11:45
centrifugation in greiner tubes
pellet stored at -20 degree Celcius, 50 ml greiner tubes
Miniprep following the Gene Jet Fermentas protocol
- pet28
- standard 10 stock 1
- standard 10 stock 7
