Team:British Columbia/NotebookKillSwitches

From 2012.igem.org

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(Reorganized into table, half-finished)
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{{Template:Template_HD_1}}
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Jacob: Odd years
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John: Even Years
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[http://ung.igem.org/Previous_iGEM_Competitions Previous iGEM Competitions]
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Team Abstracts: [https://2011.igem.org/Jamboree/Team_Abstracts 2011] [https://2010.igem.org/Jamboree/Project_Abstract/Team_Abstracts 2010]
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[https://2009.igem.org/Jamboree/Project_Abstract/Team_Abstracts 2009]
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[https://2008.igem.org/Jamboree/Project_Abstract/Team_Abstracts 2008]
Parts Registry: [http://partsregistry.org/Cell_death Cell Death]
Parts Registry: [http://partsregistry.org/Cell_death Cell Death]
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{| border="1"
{| border="1"
|+ Killswitch BioBricks
|+ Killswitch BioBricks
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! scope="col" | Number
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! scope="col" | Numbers
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! scope="col" | Name
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! scope="col" | Team
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! scope="col" | Year
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! scope="col" | Trigger
! scope="col" | What it Does
! scope="col" | What it Does
|-
|-
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| <partinfo>BBa_I716462</partinfo> || || Arabinose promoter, stops reproduction, no lysis
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| <partinfo>BBa_I716462</partinfo> || || || Arabinose promoter || Stops reproduction, no lysis
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|-
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| <partinfo>BBa_K512002</partinfo> || || Finds GFP on plasmid, eats plasmid? (Jacob says mention "crispr")
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| <partinfo>BBa_K512002</partinfo> || || || Finds GFP on plasmid || Eats plasmid? (Jacob says mention "crispr")
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|-
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| <partinfo>BBa_K112808</partinfo> || ||
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| <partinfo>BBa_K112808</partinfo> || || || ||
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|-
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| <partinfo>BBa_K541545</partinfo> || || || || Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
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|-
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| <partinfo>BBa_K593009</partinfo> || || || || ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
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|-
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| <partinfo>BBa_K628006</partinfo> || || || || Protegrin-1 Kill Switch, (Antimicrobial peptide)
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[https://2010.igem.org/Team:HKU-Hong_Kong/Project 2010 Hong Kong]: Possible new killswitch, not implemented
[https://2010.igem.org/Team:HKU-Hong_Kong/Project 2010 Hong Kong]: Possible new killswitch, not implemented
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<partinfo>BBa_K541545</partinfo>
 
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Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
 
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<partinfo>BBa_K593009</partinfo>
 
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ROSE regulated kill switch
 
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(RNA thermometer, only activates above 42°)
 
   
   
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<partinfo>BBa_K628006</partinfo>
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Protegrin-1 Kill Switch
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(Antimicrobial peptide)
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<partinfo>BBa_K565001</partinfo>
<partinfo>BBa_K565001</partinfo>

Revision as of 20:00, 30 May 2012

British Columbia - 2012.igem.org

Jacob: Odd years

John: Even Years

[http://ung.igem.org/Previous_iGEM_Competitions Previous iGEM Competitions]

Team Abstracts: 2011 2010 2009 2008

Parts Registry: [http://partsregistry.org/Cell_death Cell Death]

Killswitch BioBricks
Numbers Team Year Trigger What it Does
<partinfo>BBa_I716462</partinfo> Arabinose promoter Stops reproduction, no lysis
<partinfo>BBa_K512002</partinfo> Finds GFP on plasmid Eats plasmid? (Jacob says mention "crispr")
<partinfo>BBa_K112808</partinfo>
<partinfo>BBa_K541545</partinfo> Limulus Anti-Lipopolysaccharide Factor for E.coli (IPTG Inducible)
<partinfo>BBa_K593009</partinfo> ROSE regulated kill switch, (RNA thermometer, only activates above 42°)
<partinfo>BBa_K628006</partinfo> Protegrin-1 Kill Switch, (Antimicrobial peptide)



2010 Strasbourg: I don't know what the heck

2011 Tokyo Metropolitan: Killer Bee e.coli?

2011 UNIST Korea: Killed by high and low temps, not if dark.

2010 Harvard: Barnase cleaves RNA!

2010 Hong Kong: Possible new killswitch, not implemented



<partinfo>BBa_K565001</partinfo> <partinfo>BBa_K565002</partinfo> Colicin G,H gene cluster

http://partsregistry.org/wiki/index.php?title=Part:BBa_K565004 Microcin C51 production device

http://partsregistry.org/wiki/index.php?title=Part:BBa_K185004 RelE toxin+Double terminator

Also, check out https://2009.igem.org/Team:UNICAMP-Brazil/Yeastguard. All sorts of kill switches, but may not all work in E. coli.