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Team:Wageningen UR/Journal/week15
From 2012.igem.org
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== Lab work == | == Lab work == | ||
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| + | New electrocompetent DH5 Alfa and Mach1 ''E. coli'' cells were prepared. Transformation efficiency was determined: | ||
| + | DH5 Alfa: 10^8 | ||
| + | Mach1: 10^9 | ||
'''TuYV''' | '''TuYV''' | ||
Revision as of 13:54, 18 August 2012
week 15: 6 august - 12 august
Office work
Lab work
New electrocompetent DH5 Alfa and Mach1 E. coli cells were prepared. Transformation efficiency was determined: DH5 Alfa: 10^8 Mach1: 10^9
TuYV
- Bricking:
Last week's minipreps were digested (EcoRI, PstI), the inserts ligated into the chloramphenicol backbone from BBa_J04450 and transformed into our electrocompetent DH5 Alfa E. coli. A PCR check gave negative results, but miniprep was performed anyway.
- Expression:
Last week's minipreps were digested (XbaI, Spe1), the inserts ligated into the promotor&RBS-containing backbone from BBa_J04500 and transformed into our electrocompetent BL21 E. coli. Results were unsatisfying at first, so the entire procedure was done again on saturday.
Written by: Wouter
