"
Team:Wageningen UR/Journal/week15
From 2012.igem.org
(Difference between revisions)
m (→Lab work) |
|||
| Line 15: | Line 15: | ||
* Expression: | * Expression: | ||
| - | Last week's minipreps were digested (XbaI, Spe1), the inserts ligated into the promotor&RBS-containing backbone from BBa_J04500 and transformed into our electrocompetent BL21 ''E. coli''. Results were unsatisfying | + | Last week's minipreps were digested (XbaI, Spe1), the inserts ligated into the promotor&RBS-containing backbone from BBa_J04500 and transformed into our electrocompetent BL21 ''E. coli''. Results were unsatisfying at first, so the entire procedure was done again on saturday. |
Revision as of 13:40, 18 August 2012
week 15: 6 august - 12 august
Office work
Lab work
TuYV
- Bricking:
Last week's minipreps were digested (EcoRI, PstI), the inserts ligated into the chloramphenicol backbone from BBa_J04450 and transformed into our electrocompetent DH5 Alfa E. coli. A PCR check gave negative results, but miniprep was performed anyway.
- Expression:
Last week's minipreps were digested (XbaI, Spe1), the inserts ligated into the promotor&RBS-containing backbone from BBa_J04500 and transformed into our electrocompetent BL21 E. coli. Results were unsatisfying at first, so the entire procedure was done again on saturday.
Written by: Wouter
