Team:Wageningen UR/Journal/week10
From 2012.igem.org
(Difference between revisions)
m (→Lab work) |
|||
Line 11: | Line 11: | ||
'''TuYV''' | '''TuYV''' | ||
- | A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. Results were unsatisfying. | + | * A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. Results were unsatisfying. |
- | Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts. | + | * Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts. |
Revision as of 12:14, 18 August 2012
week 10: 2 july - 6 july
Office work
Lab work
TuYV
- A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa E. Coli was performed. Results were unsatisfying.
- Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts.
Written by: Wouter