Team:Wageningen UR/Journal/week10

From 2012.igem.org

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'''TuYV'''
'''TuYV'''
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A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. Results were unsatisfying.
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* A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. Results were unsatisfying.
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Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts.
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* Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts.

Revision as of 12:14, 18 August 2012

week 10: 2 july - 6 july

Office work

Lab work

TuYV

  • A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa E. Coli was performed. Results were unsatisfying.
  • Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts.


Written by: Wouter


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