Team:Osaka/week2

From 2012.igem.org

(Difference between revisions)
Line 38: Line 38:
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
# Gel electrophoresis of digests
# Gel electrophoresis of digests
 +
#* 1-10H not ok
# Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 3)
# Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 3)
# Transformation of plasmid DNA into Rosetta <i>E.coli</i>:147,148,150
# Transformation of plasmid DNA into Rosetta <i>E.coli</i>:147,148,150
Line 48: Line 49:
==August 10 (Fri)==
==August 10 (Fri)==
-
# Transfer to liquid culture
+
# Sequencing:143~152
 +
# Transfer to liquid culture:1-2M
 +
# Preparation of glycerol stock of cell culture:147,148,150
 +
# Transformaton of Registry parts into DH5α <i>E.coli</i>:1-10H
==August 11 (Sat)==
==August 11 (Sat)==
-
# Transfer to liquid culture
+
# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Transfer to liquid culture:1-10H
# SDS-PAGE  
# SDS-PAGE  
#* IPTG induction
#* IPTG induction
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]

Revision as of 09:38, 17 August 2012

August 5 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transfer to liquid culture
    • (Rosetta)143~146,149,152
    • (DH5α)150,Empty vector(A)

August 6 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transformaton of Registry parts into DH5α E.coli(See Table 2)
  5. Preparation of glycerol stock of cell culture:143~146,149,152
Table2
IDPart NameResistanceDescription
1-2D<bbpart>BBa_J52008</bbpart>A,K Renilla Luciferase
1-10H<bbpart>BBa_I712019</bbpart>A,K Firefly Luciferase from Photinus pyralis
1-22A<bbpart>BBa_J23118</bbpart>A Constitutive promoter family member

August 7 (Tue)

  1. Preparation of LB agar plates (Cam)
  2. Transformation of 1-2D,1-10H

August 8 (Wed)

  1. Transfer to liquid culture:1-2D,1-10H,1-22A

August 9 (Thu)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • 1-10H not ok
  4. Transformaton of Registry parts into DH5α E.coli(See Table 3)
  5. Transformation of plasmid DNA into Rosetta E.coli:147,148,150
Table3
IDPart NameResistanceDescription
1-2M<bbpart>BBa_K118022</bbpart>ARBS

August 10 (Fri)

  1. Sequencing:143~152
  2. Transfer to liquid culture:1-2M
  3. Preparation of glycerol stock of cell culture:147,148,150
  4. Transformaton of Registry parts into DH5α E.coli:1-10H

August 11 (Sat)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Transfer to liquid culture:1-10H
  4. SDS-PAGE
    • IPTG induction

Back to Notebook

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